The nucleotide sequence of potato virus A genomic RNA and its sequence similarities with other potyviruses

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Nucleotide Sequence of Potato Virus

SUMMARY The complete nucleotide sequence of the genomic RNA of the potyvirus potato virus Y strain N (PVYn) was obtained from cloned cDNAs. This sequence is 9704 nucleotides long and can encode a polyprotein of 3063 amino acids. The positions of the cleavage sites at the N terminus of the capsid and cytoplasmic inclusion proteins have been determined. Other putative protein cleavage sites have ...

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Molecular cloning of a potato virus Y genome: nucleotide sequence homology in non-coding regions of potyviruses.

An aphid-transmissible field isolate of potato virus Y, strain MM, was purified from a pepper host and cloned. All but 18 terminal nucleotides of the 9.7 kb genome are apparently contained in two overlapping cDNA clones. The library of cDNA clones is likely to be representative of the viral RNA population present in infected plants because restriction endonuclease maps derived from cloned and u...

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Nucleotide sequence of tobacco mosaic virus RNA.

Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral population. The positive strand coding sequence starts 69 nucleotides from the 5' end with a reading frame for a protein of Mr 125,941 and terminates w...

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Nucleotide sequence of narcissus mosaic virus RNA.

The nucleotide sequence of the genomic RNA of narcissus mosaic virus (NMV) was deduced from a set of cDNA clones and by direct sequencing of RNA. The genome, with a length of 6955 nucleotides [excluding the 3'poly(A) tail], contains six open reading frames (ORFs) with the capacity to code for polypeptides of more than 10K, with Mr values of 186284, 25845, 13998, 11059, 26097 and 10519. The firs...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 1994

ISSN: 0022-1317,1465-2099

DOI: 10.1099/0022-1317-75-2-457